Millpore IPVH00010 Immobilon-P PVDF Membrane, 0.45um pore size [26.5cmX3.75m] > Filter System

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Filter System

Filter System

Membrane Filters Millpore IPVH00010 Immobilon-P PVDF Membrane, 0.45um pore size [26.5cm…

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작성자최고관리자 댓글 0건 조회 559회 작성일 20-08-26 11:54

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개요

 

Replacement Information

Replacement Information

INCP 000 10 (Protein Applications)

 

주요 사양표

 

Key Applications

WB

 

Description

 

Catalogue Number

IPVH00010

Replaces

INCP00010

Trade Name

 

Immobilon

 

Description

Immobilon-P PVDF Membrane

 

Background Information

Overview

- Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.

Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.

 

Immobilon-P Transfer Membrane:

- 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa

- Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity

 

Immobilon-E Membrane:

- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.

- 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa

 

Blotting Sandwiches:


- When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.

 

Blotting Filter Paper:

- Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.

 

Immobilon-PSQ Membrane:

- 0.2 µm pore size and a large internal structure

- Higher protein adsorption and sequencing yields than other membranes

- Recommended for blotting proteins less than 20 kDa

- Prevents blow-through of low molecular weight proteins

 

Immobilon-FL Membrane:

- The first transfer membrane optimized for fluorescence applications

- Extremely low background improves sensitivity of all fluorescence detection protocols

- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths

- Ideal for multiplexing and chemifluorescence applications

 

Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.

 

Features & Benefits:

- Won’t crack, curl or fracture when cut

- Low background

- Superior staining capabilities

- Can be reprobed multiple times

 

Applications:

- Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing;

- Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection

- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths


Product Information

Detection method

Chemiluminescence Chromogenic Radioactive

Compatible Stains

Coomassie brilliant blue

Amido black

India ink

Ponceau-S red

Colloidal gold

CPTS

Toluidine blue

Transillumination

Sypro® ruby

Filter Code

IPVH

Filter Color

White

Quality Level

MQ400


Applications

Application

1 roll, 26.5 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

Key Applications

Western Blotting


Biological Information

Primary Binding Mechanisms

Electrostatic, hydrophobic

BSA Adsorption

215 µg/cm²

Goat IgG Adsorption

294 µg/cm²

Insulin Adsorption

160 µg/cm²

Wettability

Hydrophobic


Physicochemical Information

Pore Size

0.45 µm


Dimensions

Filter Surface

Plain

Filter Dimensions

26.5 cm x 3.75 m


Materials Information

Chemistry

Hydrophobic Polyvinylidene Fluoride (PVDF)



Packaging Information

Material Size

1


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